FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n01_AHPm104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n01_AHPm104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27076010
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT4025431.4867146230186796TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA919650.046.7539379
AGAGCAC928550.046.2291268
TCGTATG473650.044.33987844-45
ATCGGAA970000.044.1778952
TCGGAAG972100.044.164013
CGTATGC478350.043.99001346-47
TATGCCG479100.043.87630548-49
CTCGTAT466650.043.6199244-45
CGGAAGA986300.043.594584
GATCGGA1001900.042.7096061
TCTCGTA485500.041.95209542-43
AATCTCG486550.041.7484740-41
AAGAGCA1042500.041.2216077
ATCTCGT499700.040.5221942-43
ATGCCGT531750.039.68840848-49
GTATGCC534800.039.55111346-47
GAAGAGC1113350.038.7007946
GCCGTCT541800.038.65781850-51
CCGTCTT541400.038.6191152-53
TGCCGTC571350.036.77490650-51
Produced by FastQC (version 0.11.5)