FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n01_AHPm103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n01_AHPm103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28128883
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT4318891.5353933535149618TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA933550.049.4191139
AGAGCAC940550.048.980668
ATCGGAA959650.047.844682
TCGGAAG968850.047.4329383
CGGAAGA986600.046.6704064
GATCGGA988850.046.2678451
TCGTATG505700.044.69562544-45
TATGCCG510850.044.22820348-49
CGTATGC512100.044.21737746-47
CTCGTAT499600.043.9476544-45
AAGAGCA1061700.043.503287
TCTCGTA519100.042.27869842-43
AATCTCG518250.042.19668240-41
ATCTCGT530650.041.19280242-43
GAAGAGC1138100.040.6580056
ATGCCGT566300.040.04860348-49
GTATGCC568650.039.97892846-47
GCCGTCT571800.039.28080450-51
CCGTCTT576750.038.91320852-53
TGCCGTC605900.037.25049250-51
Produced by FastQC (version 0.11.5)