FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n01_AHPm102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n01_AHPm102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25703308
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT3743581.4564584449596916TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA840800.046.9680869
AGAGCAC845850.046.6259358
ATCGGAA859500.045.557172
TCGGAAG866550.045.2560773
CGGAAGA885500.044.3560684
TCGTATG433250.044.29258344-45
CGTATGC436650.044.13979346-47
GATCGGA885500.044.127451
TATGCCG438050.044.01555348-49
CTCGTAT428100.043.64317344-45
TCTCGTA444550.041.86941542-43
AATCTCG449100.041.3780440-41
AAGAGCA963300.041.0494967
ATCTCGT459050.040.50030542-43
GTATGCC487750.039.55438246-47
ATGCCGT489200.039.47164548-49
GCCGTCT497250.038.569550-51
CCGTCTT500600.038.3336752-53
GAAGAGC1036700.038.1524356
TGCCGTC528000.036.642850-51
Produced by FastQC (version 0.11.5)