FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n01_AHPm101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n01_AHPm101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24062625
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT3309901.3755357115027975TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG385850.043.98855244-45
CGTATGC387200.043.94683546-47
GAGCACA800200.043.7042169
TATGCCG390100.043.6078648-49
AGAGCAC803400.043.429698
CTCGTAT380350.043.34394544-45
TCGGAAG830800.041.826823
ATCGGAA832750.041.6961442
CGGAAGA840150.041.3548244
TCTCGTA398550.041.2898142-43
AATCTCG399650.041.1073940-41
GATCGGA858600.040.350161
ATCTCGT411950.039.8832842-43
GTATGCC435400.039.23460846-47
ATGCCGT436900.039.10526348-49
AAGAGCA909700.038.3861667
GCCGTCT451650.037.5909450-51
CCGTCTT452300.037.54664652-53
GAAGAGC974000.035.9690256
TGCCGTC477050.035.813650-51
Produced by FastQC (version 0.11.5)