FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32977008
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19121355.798388380170814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA17300.09.6053839
CCCGTAT22600.08.7286171
CGGGAAT38700.08.3297861
CCGGGAT42250.07.74376631
CGGGTAT21800.07.7246991
CGGGGAT57400.07.6278041
CTACCGT19600.07.50981768
GGGGAAT82750.07.15166431
CTGGAAT142100.07.0765611
CTCGGGT46000.07.00788551
CGGGATT31600.07.00391341
CTCGGAT39250.06.49690061
CGGAAAT47500.06.4827071
CGTATCA37100.06.462810550-51
GCCGTAT33900.06.44299148-49
CTCGAAT44600.06.36483531
GATCGGA252250.06.253696494-95
AGATCGG274400.06.198425394-95
CTCGGTT31300.06.14873361
CCGGAAT35250.06.14219241
Produced by FastQC (version 0.11.5)