FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_ALm228RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_ALm228RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38013079
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG489280.12871359355026202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA37500.013.2985559
CTACCGT48800.010.8025898
CGCGGGT72900.010.3665151
CGCGAAT22200.09.2061631
CGGGGAT118050.08.4953181
CGGGTAT37900.08.1514961
GGGGAAT198250.08.0314661
GTCCGAT31400.08.0224941
ACTACCG65300.08.000217
GCGTCGT309750.07.885829494-95
GATCGGA718500.07.839967394-95
GTCGGAT41000.07.6510891
CCGGGAT100200.07.54210571
ATCGGAA675750.07.362893694-95
AGATCGG802450.07.35996494-95
AGCGTCG359850.07.35523794-95
CGTCGTG302250.07.09193594-95
AGAGCGT430500.06.96423694-95
CGGGTCT136850.06.9049033
CGACCTA23750.06.7979644
Produced by FastQC (version 0.11.5)