FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_ALm227RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_ALm227RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37492746
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG466520.12442940295704133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA35350.010.8828619
CCGGGAT106400.09.11307051
CTACCGT47700.08.9610148
CGCGGGT74950.08.8783581
CGGGGAT118300.08.0758371
CGCGTTT27300.07.48655561
GGGGAAT188700.07.45583061
CGGGAAT85150.07.424091
GATCGGA657350.07.42070194-95
GTCGGAT39850.07.3950241
ATCGGAA624850.07.30901994-95
CGCCAAT68350.07.232211
CCGGAAT82450.07.2060221
CGGGGTT116850.07.1998061
ACTACCG63850.07.06632147
CGGGTAT41150.07.0458961
CGTCGTG287850.07.042412394-95
AGATCGG736100.07.029905394-95
GCGTCGT289250.07.00832694-95
CGACCGT29000.06.8837881
Produced by FastQC (version 0.11.5)