FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_ALm226RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_ALm226RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33520454
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG392020.11694949000392418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA35650.010.924469
CGGGGAT100250.09.1981711
CGGGTAT34900.09.1250141
CTACCGT46600.08.9685388
CGCGGGT61700.08.3970261
CGGGAAT80250.07.9367721
CCGGGAT92400.07.8705241
GGGGAAT174300.07.85378931
CGGGGTT106150.07.83614061
CTCGGAT92400.07.66475871
CGCGTAT7452.1269475E-47.6561271
CGACCTA20750.07.5518274
TCGGATT56200.07.5228712
CCCGTAT51600.07.46138531
CGGGATT56850.07.2739941
GCGTCGT271500.07.265422394-95
CGTATCG39900.07.25942333
AGCGTCG307300.07.106486394-95
AGATCGG673450.07.0459194-95
GATCGGA600900.07.02359194-95
Produced by FastQC (version 0.11.5)