FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_ALm225RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_ALm225RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34228003
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG367310.10731271701711607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA34500.011.9768389
CGCGGGT62200.010.4687491
CTACCGT46800.09.9449798
CGGGGAT107500.09.7712121
CGGGGTT106200.08.3691571
CGGGAAT81150.07.9655361
CGACCGT27050.07.9069661
GGGGAAT180950.07.801221
ACTACCG60900.07.56435257
GCGTCGT283250.07.525357294-95
CCGGGAT91250.07.500571
TCGGATT54650.07.4751212
GATCGGA650800.07.331101494-95
CGCGGAT41150.07.2767151
CGGGTAT34300.07.2056621
CGGCGAT54550.07.14469581
CGTCGTG279900.07.132040594-95
ATCGGAA617750.07.123897694-95
AGCGTCG326700.06.945914794-95
CGAGAAT74700.06.93538431
Produced by FastQC (version 0.11.5)