FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_ALm220RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_ALm220RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40304763
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG581780.14434522292067564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT78700.010.3874341
TACCGTA41350.09.9929879
CGGGGAT126500.08.5664121
CTACCGT53350.08.1018
GCGTCGT300500.08.07339394-95
GGGGAAT192500.07.802091
GATCGGA687800.07.624005394-95
CGCGAAT25950.07.5093291
GTATCGA55200.07.48415574
AGATCGG777700.07.35011394-95
AGCGTCG343800.07.33277494-95
CGACCGT33450.07.24650671
ATCGGAA651500.07.225334694-95
CCGGGAT110800.07.20649621
CCCGTAT60850.07.1859121
CGGGGTT127300.07.13115021
CGTATCG43250.06.91683
CGCGTAT9002.4642618E-46.8652361
CGCCAAT76300.06.85208561
GGCGAAT54250.06.8335991
Produced by FastQC (version 0.11.5)