FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_ALm219RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_ALm219RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39479965
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG564250.14292059276141708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA36250.09.5643829
CGCGGGT78650.09.4887991
CGGGTAT43950.08.6524961
CGCGTAT9407.7388177E-78.0910041
CGGGGAT123900.07.94162461
CGGGGTT122100.07.5915291
CTACCGT48400.07.55562358
CCCGTAT58600.07.54389521
CGTATCG42300.07.52115543
CCGGGAT107050.07.37109041
CGGGAAT89200.07.3540161
GATCGGA689600.07.321830394-95
CGGCGAT69250.07.20742031
AGATCGG775000.07.164369694-95
CGCGAAT24750.07.1061911
ATCGGAA659100.07.059179394-95
GGGGAAT197300.07.0591251
GCGTCGT303650.07.01243194-95
CGGGATT65900.06.99675461
TCGGATT63750.06.9301342
Produced by FastQC (version 0.11.5)