FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_ALm218RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_ALm218RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33310492
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG595390.17873947944089208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT64950.010.3183591
TACCGTA33300.09.9836619
CGGGTAT35200.09.857141
CTACCGT41700.09.4527998
CGGGGAT103800.08.8375251
CGGGGTT104850.07.70639371
CGGGAAT74500.07.65589051
CCCGTAT48100.07.5099881
GGGGAAT162800.07.41566371
CTCGGTT62200.07.4122841
CGTATCG38100.07.2284383
CCGGGAT89600.06.94918731
CGGGTCT129200.06.9093433
AATCGCG9901.0919371E-46.7156255
CGCGAGT34750.06.70211651
CTCGGAT90900.06.64064931
GTCGGAA71300.06.59958121
CGGCGAT57450.06.53593541
ACTACCG55450.06.5092037
GGGAAAT267950.06.47455361
Produced by FastQC (version 0.11.5)