FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_ALm217RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_ALm217RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34074542
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG426080.12504350021784594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT64850.010.5538381
TACCGTA32400.09.381779
CGCGATT10103.1523086E-98.9410831
GTCGGAT35200.08.5065961
CTACCGT43250.08.2358388
CGGGGAT104550.08.0919631
GGGGAAT164750.07.87580821
CCCGTAT48550.07.8317471
CGGGTCT125650.07.78474243
TCGGATT57650.07.6630172
CCCGGAT109350.07.5194351
CTCGGGT86300.07.49007231
CGGGTAT37700.07.43821241
CGGGAAT79400.07.4226521
GCGTCGT281500.07.42066994-95
CGCGTAA7100.00107004757.3636351
CGCGGAT42900.07.3121411
GTCCGAT29800.07.1771851
CCGGGAT91950.07.13321451
CGCGAAT20000.07.1293371
Produced by FastQC (version 0.11.5)