FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_ALm212RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_ALm212RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32430057
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG368060.1134934792128179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA29850.010.9789679
CGCGGGT68400.010.0765941
CTACCGT40400.08.6994088
CGGGGTT101750.08.595781
CGGGGAT107950.08.4103211
GGGGAAT167150.08.3322671
GCGTCGT249600.07.884258794-95
CGGGTAT38000.07.7555031
CGCGAAT17900.07.7009951
GATCGGA596450.07.53403394-95
CTCGGAT91550.07.52855351
CCGGGAT90450.07.40990161
CGACCGT25750.07.38388061
CCCGTAT46400.07.3759241
CGTCGTG246000.07.33380694-95
ATCGGAA565450.07.321552894-95
CGTATCG35100.07.30538133
CTCGTAT33850.07.30207971
AGATCGG668950.07.27818294-95
GGCGAAT43400.07.22863151
Produced by FastQC (version 0.11.5)