FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_ALm211RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_ALm211RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39719951
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG439660.11068996535267628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT79500.09.3866411
TACCGTA36550.09.2259849
CGCGTAT9205.975562E-88.7829171
CGGGGAT124550.08.5101811
CCGGGAT113800.08.061071
CGGGAAT91150.07.92619231
GGGGAAT204500.07.90247731
CTACCGT48300.07.7679098
GCGTCGT326450.07.642483794-95
GTCGGAT42000.07.5823391
GATCGGA741850.07.24130694-95
CGGGGTT130000.07.2393491
ATCGGAA705700.07.181682694-95
CGGGTAT39150.07.16304871
CTCGGTT72350.07.1608671
AGATCGG826600.07.041635594-95
CGCGGAT51100.06.97618531
CGTATCG44700.06.90472463
AGCGTCG374800.06.90359694-95
CGTCGTG325400.06.901159394-95
Produced by FastQC (version 0.11.5)