FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_ALm210RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_ALm210RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31952817
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG351780.11009357954261123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA32100.011.6887759
CGCGGGT50550.09.6851391
CTACCGT42100.09.4761198
CGGGGAT92000.09.4548151
CGGGTAT30450.09.0537911
CTCGTAT33500.08.229491
CCGGGAT80100.08.1297621
CTCGGAT91650.07.9350331
GCGTCGT287250.07.858824794-95
GGGGAAT161100.07.7302851
CCCGTAT51700.07.447051
ACTACCG53150.07.41661027
GATCGGA683400.07.394993394-95
ATCGGAA648650.07.37763694-95
CGGGAAT73600.07.3623561
CGTATCG39450.07.34226133
CGGGGTT92600.07.34031721
CGTCGTG282150.07.33624194-95
AGCGTCG328300.07.288308694-95
GTCCGAT27650.07.2201121
Produced by FastQC (version 0.11.5)