FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_ALm209RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_ALm209RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31544200
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG346870.1099631628001344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT7351.5152182E-910.9937961
CCGGGAT88050.09.7709171
CGGGGAT94300.09.6273721
CGCGGGT59200.08.831961
TACCGTA29950.08.7217589
CGGGAAT74000.07.77212431
CCCGTAT49050.07.7524151
CGGGGTT98300.07.44651841
GCGTCGT272850.07.377670394-95
CGCGATT11251.4874659E-67.18261341
AGAGCGT380850.07.16788894-95
GATCGGA630300.07.16702394-95
GGGGAAT160500.07.1372041
CGGGATT51650.07.08608681
CGTCGTG271800.07.074290894-95
AGCGTCG313250.07.055145394-95
CTCGGTT54950.07.00653651
CGCGAAT17651.0186341E-107.0018811
CGCGTAA8155.968547E-46.9985771
CGTATCG37350.06.99225473
Produced by FastQC (version 0.11.5)