FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_ALm204RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_ALm204RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31852793
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG369960.11614680069028796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA30350.010.0153059
CGCGTAA8652.1966116E-89.34147551
CGCGGGT63050.08.4433661
CCGGGAT87500.08.3655671
CGCGTAT7402.1704553E-58.350151
CGGGTAT32600.08.3107451
CTACCGT40100.08.0536058
CTCGGTT60800.08.0522331
CGGGATT54800.07.8062891
CGGGGAT104200.07.70906451
CTCGTAT32250.07.66401431
GCGTCGT261400.07.61007694-95
GGGGAAT163350.07.5363891
GATCGGA618950.07.517112794-95
CTCGGAT88150.07.49506041
CGGGGTT101350.07.40996271
ATCGGAA589400.07.40665694-95
CGCGAAT17359.094947E-127.3968531
AGCGTCG300850.07.35387994-95
CCGGAAT76050.07.3125581
Produced by FastQC (version 0.11.5)