FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_ALm203RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_ALm203RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36469670
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG421760.1156467826552859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA36100.08.94640359
CGCGGGT73250.08.8898061
CGGGGAT115950.08.5675151
CCGGGAT104050.08.4966981
CGGGATT66700.08.1950451
CGGGAAT82900.08.14166451
CGGGTAT40900.08.1349341
CTACCGT43700.08.04233558
CGACCGT30800.08.0247591
GGGGAAT194750.07.907641
CGGGGTT114500.07.80426031
GCGTCGT271700.07.443753294-95
CCCGGAT120650.07.28825861
CGCTAAT19001.8189894E-127.25477171
CTCGGTT67600.07.242191
GATCGGA622400.07.16636994-95
ATCGGAA593350.07.161171494-95
CGCGATT12055.7919533E-77.10010531
CTCGTAT38250.07.083091
AGATCGG698450.07.05994-95