FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_ALm202RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_ALm202RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32681883
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG441640.13513297260136448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT71650.08.7567621
CGCGTAA10405.326001E-98.6837241
TACCGTA32550.08.4628059
GGGGAAT174250.08.2379661
CCCGTAT48200.08.0863511
CTCGGAT90150.08.0142641
CGCGAAT18050.07.900051
CCGGGAT91550.07.78787041
CTACCGT41500.07.781738
GTCCGAT29050.07.6901981
CGTATCG35350.07.6565483
TCGGATT52600.07.5863922
GCGTCGT271000.07.48924994-95
CGGGGAT104800.07.3474991
ATCGGAA598250.07.308839394-95
GATCGGA626800.07.19558594-95
CGGGGTT104650.07.04009061
CGGGAAT72950.07.0369471
CGGGTAT37350.06.99935251
CGTCGTG272700.06.97250594-95
Produced by FastQC (version 0.11.5)