FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n02_ALm201RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n02_ALm201RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31820489
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG361550.11362176112378411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT90600.08.8663041
CCGGGAT90000.08.8197881
TACCGTA31750.08.3769049
CGCGGGT60300.08.276681
CGGGGTT99300.08.23311
CGCGATT9952.1103006E-78.1210091
GGGGAAT159550.07.8350741
CTACCGT40550.07.49560178
GATCGGA532000.07.304324694-95
CTCGGAT88700.07.28785041
GCGTCGT237550.07.254785594-95
AGATCGG597300.07.22510894-95
CGTATCG33000.07.19446233
TCGGATT55600.07.17687232
CCCGTAT45850.07.0494261
CGTCGTG235300.07.011418394-95
ATCGGAA506950.06.902006694-95
CGGGTAT33950.06.86025861
CGCCAAT54300.06.8277711
AGCGTCG270950.06.798538794-95
Produced by FastQC (version 0.11.5)