FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32977008
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG454840.13792640011489218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA87950.020.63395142-43
ATGCCGT111250.018.9188748-49
CTCGTAT79600.018.35240244-45
CGCGAAA34350.017.35485836-37
AGAGCAC392100.015.9899018
TCGGAAG390750.015.79282953
ATCGGAA402100.015.6903092
CGGAAGA408550.015.4065254
CGTCTGA205700.015.29862316-17
ACGTCTG195300.015.22633914-15
TAATGCG33950.015.04214334-35
CACGTCT213400.014.90165914-15
GATCGGA407750.014.7154851
ATCTCGT99600.014.45234142-43
CCGCGAA47950.014.36548934-35
ACACGTC215850.014.06181512-13
CACACGT236350.013.68491812-13
GAGCACA490400.013.17159759
GCGAAAT52050.012.86774536-37
TCCGCGA46950.012.74899634-35
Produced by FastQC (version 0.11.5)