FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_ALm228RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_ALm228RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38013079
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT742270.1952670027071472TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA155100.035.7518336-37
CGTATGC126800.034.57615746-47
TATGCCG131750.033.7281848-49
TCGTATG123500.033.69118544-45
CTCGTAT126750.031.57192844-45
GCGAAAT181900.030.0013336-37
CCGCGAA187250.029.60108434-35
TCCGCGA197850.028.42328334-35
CGAAATC181350.027.35526338-39
TCTCGTA148950.027.2327642-43
ACTCCGC216450.025.96973632-33
AATCTCG167750.025.36994440-41
ATCTCGT172350.024.36213142-43
CACTCCG240250.023.24876830-31
CTCCGCG246950.022.74306532-33
ATGCCGT195650.022.1661748-49
GTATGCC210900.021.49778246-47
CGCGTAT14950.020.33377344-45
GCCGTCT205850.019.6487150-51
CCGTCTT213600.019.54728752-53
Produced by FastQC (version 0.11.5)