FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_ALm227RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_ALm227RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37492746
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT668130.17820247148608429TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA136950.034.5599336-37
CGTATGC115300.032.81352246-47
TCGTATG112100.032.5624444-45
TATGCCG117300.032.4164348-49
CTCGTAT115150.030.50373344-45
GCGAAAT161350.028.92152636-37
CCGCGAA169000.027.89384334-35
TCCGCGA176300.027.26419834-35
CGAAATC165050.025.88441338-39
TCTCGTA138400.025.5163442-43
AATCTCG148800.024.96193140-41
ACTCCGC199900.024.17572432-33
ATCTCGT160850.023.16569742-43
CACTCCG225000.021.33110630-31
CTCCGCG233650.020.54132332-33
ATGCCGT183000.020.47989848-49
GTATGCC193250.020.22910746-47
CCGTCTT202450.017.83182152-53
GCCGTCT197350.017.78726650-51
CGCGTAT13300.017.1422744-45
Produced by FastQC (version 0.11.5)