FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_ALm226RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_ALm226RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33520454
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT852990.25446851048019814TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA160400.037.6054536-37
CGTATGC133400.036.19487446-47
TCGTATG128550.035.6933744-45
TATGCCG138050.035.0967648-49
CTCGTAT128650.034.26263444-45
CCGCGAA186950.032.04940834-35
GCGAAAT186000.031.91894736-37
TCCGCGA196400.031.0998134-35
CGAAATC182300.029.59642238-39
TCTCGTA151750.028.96841642-43
AATCTCG163400.028.25469440-41
ACTCCGC217100.028.25441732-33
ATCTCGT174900.026.43767242-43
CACTCCG239350.025.44919830-31
ATGCCGT192100.024.7396248-49
CTCCGCG246500.024.65330532-33
GTATGCC207700.023.63575646-47
CGCGTAT14600.022.77339244-45
CCGTCTT205800.022.4579552-53
GCCGTCT202250.021.86580350-51
Produced by FastQC (version 0.11.5)