FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_ALm225RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_ALm225RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34228003
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT813900.23778775524823928TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA156050.036.26462636-37
CGTATGC130000.034.87608346-47
TCGTATG126000.034.36081744-45
TATGCCG134650.034.00727548-49
CTCGTAT128050.032.47535744-45
CCGCGAA180200.031.29952834-35
GCGAAAT182350.030.73471636-37
TCCGCGA191200.029.99564634-35
CGAAATC178100.028.61425438-39
TCTCGTA148600.028.0957242-43
AATCTCG158400.027.69185840-41
ACTCCGC213850.026.91829532-33
ATCTCGT172400.025.3328842-43
CGCGTAT12900.024.4855244-45
CACTCCG233800.024.3166230-31
CTCCGCG238650.023.93193632-33
ATGCCGT192500.023.38027648-49
GTATGCC203150.022.94925746-47
CCGTCTT197150.021.8288152-53
AATCGCG18850.021.0399940-41
Produced by FastQC (version 0.11.5)