FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_ALm220RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_ALm220RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40304763
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT597060.14813633812956548TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG113700.031.37344444-45
CGTATGC118550.030.81169346-47
TATGCCG120750.030.32937248-49
CTCGTAT114650.028.17199144-45
TCTCGTA136850.022.83794642-43
CGGCTAT173400.021.05047434-35
CCGGCTA179750.019.93666632-33
ATCTCGT158550.019.80209742-43
ATGCCGT192700.018.88179248-49
GTATGCC200200.018.43519646-47
ACCGGCT208300.017.83116532-33
TCACCGG210000.017.60765630-31
CCGTCTT214300.017.36648652-53
GCCGTCT212450.017.21591850-51
GTCACCG221200.016.43661328-29
TATGATC200500.016.10821238-39
TGCCGTC248250.015.11589950-51
CGTCTTC272650.013.65858452-53
GATCTCG236000.013.49474340-41
GCTATGA279600.013.428390536-37
Produced by FastQC (version 0.11.5)