FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_ALm219RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_ALm219RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39479965
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT638570.16174533082792755TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG118500.032.78785344-45
CGTATGC123300.032.1867946-47
TATGCCG126650.031.39215948-49
CTCGTAT118700.029.3513144-45
TCTCGTA141500.023.76570942-43
CGGCTAT181250.021.7893334-35
CCGGCTA187400.020.88401432-33
ATCTCGT160150.020.74601742-43
ATGCCGT193800.020.40475348-49
GTATGCC208550.019.0296446-47
CCGTCTT215350.018.8811552-53
TCACCGG215000.018.65594530-31
GCCGTCT220050.018.26208950-51
ACCGGCT221600.018.05745332-33
GTCACCG227050.017.43547628-29
TATGATC206300.016.5300938-39
TGCCGTC247450.016.45109650-51
CGTCTTC269850.014.97102252-53
GATCTCG235150.014.43210140-41
GCTATGA282700.014.09583736-37
Produced by FastQC (version 0.11.5)