FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_ALm218RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_ALm218RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33310492
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT684070.20536172206642878TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC116650.034.93770246-47
TCGTATG115550.034.6116944-45
TATGCCG117650.034.54024548-49
CTCGTAT117200.031.1456244-45
TCTCGTA138750.025.65748842-43
CGGCTAT165300.024.86890834-35
CCGGCTA164400.024.36930532-33
ATCTCGT151300.023.5606542-43
ATCGGAA358850.022.8440972
TCACCGG181850.022.77519230-31
GATCGGA359750.022.4721531
ATGCCGT185650.022.0423448-49
GTATGCC186000.021.92396746-47
ACCGGCT190350.021.8330832-33
GTCACCG188050.021.7333328-29
TCGGAAG390450.021.056063
GCCGTCT198700.020.51101150-51
CCGTCTT200750.020.5026252-53
TATGATC187450.019.2311738-39
CGGAAGA436300.018.9522044
Produced by FastQC (version 0.11.5)