FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_ALm217RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_ALm217RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34074542
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT1046730.3071882815035342TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG161700.037.89306644-45
CGTATGC165550.037.4432246-47
TATGCCG168250.036.65935548-49
CTCGTAT157150.035.54454844-45
TCTCGTA177050.030.62350842-43
CGGCTAT217950.028.60266934-35
CCGGCTA223650.027.79903832-33
ATCTCGT195300.027.57946242-43
ATGCCGT228250.027.07474748-49
GTATGCC235150.026.46172746-47
ACCGGCT247000.025.34413732-33
TCACCGG248550.025.33884830-31
GCCGTCT246150.025.31818250-51
CCGTCTT244950.025.30641752-53
GTCACCG257250.024.20434428-29
TATGATC236450.023.13997838-39
TGCCGTC272200.022.9649950-51
CGTCTTC291300.021.45102152-53
GATCTCG262000.020.82116340-41
GCTATGA304300.020.53269836-37
Produced by FastQC (version 0.11.5)