FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_ALm212RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_ALm212RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32430057
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT495920.15291986690001808TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC90900.032.5291546-47
TCGTATG85050.032.44760544-45
TAATGCG120250.031.69762434-35
TATGCCG95600.031.60119448-49
CTCGTAT93400.029.54677644-45
AATGCGC141700.026.69824434-35
TCTCGTA114450.023.82156242-43
ATGCGCA171400.022.3349336-37
TGCGCAT165100.022.18031536-37
CGCGTAT14500.021.7838444-45
ATCTCGT134850.021.3449842-43
GCGCATC163600.021.07688138-39
CTAATGC195000.019.66836732-33
ATGCCGT152050.019.38472448-49
GTATGCC159000.019.2540946-47
CGCATCT172400.017.7144138-39
GTCACTA218950.017.69014528-29
ATCGCGT19500.017.17230642-43
GCCGTCT163800.016.83421550-51
ACTAATG233500.016.7610332-33
Produced by FastQC (version 0.11.5)