FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_ALm211RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_ALm211RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39719951
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT621110.15637229763954139TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG102350.030.88449344-45
TATGCCG112800.030.23540948-49
TAATGCG139350.030.18193434-35
CGTATGC111950.029.89173346-47
CTCGTAT107550.028.9716844-45
AATGCGC162250.025.79031834-35
TCTCGTA135700.022.96133642-43
ATGCGCA200450.021.23049236-37
ATCTCGT155550.020.88619242-43
TGCGCAT195950.020.6515436-37
GCGCATC197900.019.43975638-39
CGCGTAT15500.018.84619544-45
ATGCCGT180450.018.61071248-49
CTAATGC230800.018.30508832-33
GTATGCC192200.018.2759146-47
CGCATCT206650.016.70900338-39
GTCACTA262250.016.3992628-29
CCGTCTT197100.015.92990452-53
GCCGTCT196250.015.76903950-51
ACTAATG275400.015.60796732-33
Produced by FastQC (version 0.11.5)