FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_ALm210RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_ALm210RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31952817
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT615260.192552662884152TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG100250.033.26085744-45
TATGCCG109100.033.1116448-49
CGTATGC108000.033.09645546-47
TAATGCG138400.032.8598134-35
CTCGTAT108250.030.78084244-45
AATGCGC156950.028.50702934-35
TCTCGTA129550.025.3161842-43
TGCGCAT179150.024.37751836-37
ATGCGCA187800.024.26632136-37
ATCTCGT145450.023.67533742-43
GCGCATC178500.023.36173438-39
CGCGTAT15550.022.90928644-45
GTATGCC174650.020.98292746-47
CTAATGC217400.020.92962832-33
ATGCCGT170050.020.54528848-49
CCGTCTT173050.019.900852-53
CGCATCT189200.019.42981138-39
GTCACTA237350.019.4000128-29
GCCGTCT171400.019.24720450-51
ATCGCGT20500.018.53565642-43
Produced by FastQC (version 0.11.5)