FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_ALm209RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_ALm209RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31544200
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT629630.1996024625763215TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG101700.033.74405744-45
CGTATGC107200.033.69767846-47
TATGCCG109450.033.3310748-49
TAATGCG142150.032.57786634-35
CTCGTAT109100.031.12874844-45
AATGCGC161850.028.53920234-35
TCTCGTA127700.026.63139342-43
ATGCGCA191950.024.17483136-37
ATCTCGT145550.024.16487942-43
TGCGCAT188850.023.71655836-37
CGCGTAT13800.022.716744-45
GCGCATC188100.022.34617638-39
CTAATGC214650.021.67374632-33
ATGCCGT168050.021.3267448-49
GTATGCC179150.021.06554246-47
GTCACTA236000.019.70241228-29
CCGTCTT176450.019.55766752-53
CGCATCT194200.019.35755238-39
GCCGTCT173300.019.1594550-51
ACTAATG248000.019.12303532-33
Produced by FastQC (version 0.11.5)