FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_ALm204RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_ALm204RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31852793
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT513300.1611475640456396TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC91100.032.32742346-47
TCGTATG90850.031.8924644-45
TATGCCG92850.031.74415648-49
CTCGTAT96650.028.60251644-45
TATCTCG107250.024.71224440-41
TCTCGTA116950.022.96717842-43
ATCTCGT130000.021.02699942-43
ATGCCGT156450.018.7939948-49
GTATGCC160600.018.61863946-47
GCTATCT146350.018.38468738-39
CCGTCTT170900.017.66341452-53
GCCGTCT168100.017.66103750-51
AGCTATC172000.016.15386438-39
TGCCGTC201050.015.10916350-51
CTATCTC190800.014.5755140-41
CGTCTTC211650.014.12793352-53
AAGCTAT223550.013.19389736-37
GAAGCTA236600.013.10854836-37
TACCGTA30950.012.2763099
AGTCACC287350.010.97528-29
Produced by FastQC (version 0.11.5)