FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_ALm203RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_ALm203RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36469670
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT639920.17546635327383TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG107250.033.63630744-45
CGTATGC114400.032.490346-47
TATGCCG115600.032.07135448-49
CTCGTAT114700.030.3541744-45
TATCTCG128050.026.41043540-41
TCTCGTA139200.024.2608242-43
ATCTCGT156800.022.00719642-43
GTATGCC187950.019.75067946-47
ATGCCGT188800.019.47337548-49
GCTATCT179550.018.926638-39
GCCGTCT203050.018.37579250-51
CCGTCTT210250.018.00616852-53
AGCTATC202500.017.26239838-39
TGCCGTC238850.015.98945450-51
CTATCTC222750.015.7686940-41
CGTCTTC258500.014.58093552-53
AAGCTAT258600.014.43606836-37
GAAGCTA273300.014.18095336-37
GAGCACA645150.012.0511949
ACGTCTG312350.011.82986714-15
Produced by FastQC (version 0.11.5)