FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_ALm202RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_ALm202RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32681883
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT575680.17614652130050157TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG97300.033.36608544-45
CGTATGC102900.032.1973946-47
TATGCCG104400.031.530648-49
CTCGTAT102800.030.19479244-45
TATCTCG111500.026.79493340-41
TCTCGTA125450.024.11822542-43
ATCTCGT138650.021.89059342-43
ATGCCGT164850.020.27097548-49
GTATGCC167650.019.8895746-47
GCCGTCT176700.018.79060650-51
GCTATCT161200.018.65053638-39
CCGTCTT186050.018.1526152-53
AGCTATC184600.016.86528238-39
TGCCGTC209550.016.1962650-51
CTATCTC201350.015.34520140-41
CGTCTTC231300.014.66296352-53
AAGCTAT235050.014.06389436-37
GAAGCTA247150.014.01913836-37
ACGTCTG300750.011.14133814-15
GAGCACA627150.011.0869579
Produced by FastQC (version 0.11.5)