FastQCFastQC Report
Thu 3 Oct 2019
HJ732BGXC_n01_ALm201RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ732BGXC_n01_ALm201RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31820489
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT548150.1722632232333073TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG93100.032.62610644-45
CGTATGC98800.031.53835546-47
TATGCCG99450.031.2611248-49
CTCGTAT100000.029.42494644-45
TATCTCG107450.026.52245940-41
TCTCGTA121000.024.02331542-43
ATCTCGT135750.021.4655342-43
ATGCCGT157600.019.71156948-49
GTATGCC161500.019.5146446-47
GCTATCT150500.018.99823438-39
CCGTCTT168050.018.65536352-53
GCCGTCT167200.018.46619450-51
AGCTATC175600.016.70189338-39
TGCCGTC200300.016.031250-51
CTATCTC193700.015.21532240-41
CGTCTTC212350.014.71878352-53
AAGCTAT227350.013.74634536-37
GAAGCTA239650.013.63538236-37
ACGTCTG264850.011.90740914-15
GAGCACA555100.011.8759049
Produced by FastQC (version 0.11.5)