FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58466206
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3725560.6372159671178254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG111200.062.6246955
CGTATAC102500.061.4225465
CGTATAA195000.061.038875
GTATAGC116050.060.580116
TATACGG114350.060.4069757
CCGTATA123350.059.4902084
GCTATAC101800.059.0962375
CTATACG82200.058.5498476
GTATAGG204700.058.3685876
TATAAGG172450.058.267797
TTATACG128900.057.8242156
GTATACG113350.057.7339746
CTATACA157750.057.44836
GGGTATA184750.057.2122734
TCGTATA176000.057.1352774
CGCTATA121250.057.0300944
GTATATC151550.056.8213126
TGTATAG233150.056.766865
AGTATAG164450.056.3413545
TATACTG161450.056.258757
Produced by FastQC (version 0.11.5)