FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_9-LBD4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_9-LBD4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2089021
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG41960.20085963712188631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG44250.065.531186
GGTATAG38400.065.3122565
CTATAGG20950.064.782016
CCTATAC14800.064.758325
CGTATAG19500.064.576525
CGTATAC15750.064.18365
TATACGG25750.063.7092327
CGGTATA28500.063.698514
TTATAGC29400.063.5331956
CGCTATA20800.063.231184
CTATACC16850.062.899836
GTATAGC22100.062.677216
GCTATAG24250.062.60155
GCTATAC18000.062.5734525
GCTATAA32750.062.4814835
TATAGGC27350.062.4121677
CCTATAG13700.062.2982755
CTATAGC16350.062.2561076
CGTATAA31100.062.1972965
GATATAG30750.061.5402035
Produced by FastQC (version 0.11.5)