FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_81-BBX31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_81-BBX31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12060242
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG307120.2546549231765001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC75650.067.14825
GTATAGC112050.066.191276
CCTATAC72900.065.938035
CGTATAG111300.065.851495
GGTATAG188900.065.672495
CTATACC73050.065.084266
GTATAGG203400.064.98126
CGTATAA186800.064.6690445
GGTATAA235300.064.512595
TATAGCG122250.064.503017
GTATACG106750.064.168456
GGTATAC106850.063.977435
GTATACC67900.063.837856
GCTATAG125750.063.598225
CTATAGC90950.063.583866
TTATAGC150400.063.293266
AGTATAG154650.063.273125
TATACCG83050.063.271087
CTATACG80050.063.151446
CGGTATA156750.063.1173254
Produced by FastQC (version 0.11.5)