FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_80-LBD37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_80-LBD37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6006813
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG98060.16324796526877064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG52850.066.593655
CGTATAC38900.066.282325
CTATACC35700.065.657816
GGTATAG93300.065.657495
GTATAGC56550.065.5772256
CCTATAC38600.065.437955
GCTATAG61650.065.146135
GTATAGG95200.064.898346
CCTATAA72200.064.688035
CTATAGC45950.064.640096
CGTATAA92900.064.546835
GTATACG53550.064.285856
CTATACG41150.064.2734766
TTATAGC77500.064.236676
GTATACC33200.064.173986
GGTATAC49950.063.9462555
TATAGCG66700.063.8853457
GGTATAA109350.063.858925
GCTATAA95350.063.658825
AGTATAG77600.063.5678065
Produced by FastQC (version 0.11.5)