FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_8-HB23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_8-HB23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2883516
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG77000.26703510575283784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG30050.066.822985
CTATACC22700.066.575796
CGTATAC28400.065.77815
GTATAGC31500.065.634986
GTATAGG45600.065.286466
CTATAGC26050.064.997416
GTATACC21550.064.9338466
CGTATAA52400.064.758815
GGTATAG47100.064.469935
GTATACG35000.064.368936
CTATAGG26150.064.213746
GGTATAC33650.064.144375
GGTATAA52800.064.1357045
TTATAGC33550.063.8141986
GCTATAG32850.063.6831055
TATACGG35600.063.5788577
TATAGCC22750.063.50787
CCTATAC23800.063.4987565
GCTATAA47350.063.242965
TATACTG41150.063.165137
Produced by FastQC (version 0.11.5)