FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_79-E2F3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_79-E2F3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8955474
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG162360.181296936376567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG86400.065.920785
GGTATAG176200.065.462825
CGTATAC55450.065.3010945
GTATAGG186400.065.14646
GTATAGC90750.065.112556
CCTATAC50400.064.763875
GCTATAG106300.064.3755
CTATACC50200.063.9765246
GGTATAA180850.063.876375
TATAGCG93000.063.7622457
AGTATAG131750.063.7032055
CGTATAA129750.063.4448285
GTATACG82000.063.314286
TATACGG99450.062.9687547
CTATAGC66250.062.8409546
GGTATAC82500.062.6761325
GATATAG134350.062.2881135
CGCTATA73850.062.200794
CTATACG60200.062.008276
GGGTATA170250.061.996884
Produced by FastQC (version 0.11.5)