FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_78-BZR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_78-BZR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8637428
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG142920.165465923420722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC62700.066.954355
CGTATAG82700.066.287035
GTATAGG175800.065.927666
CCTATAC51650.065.632445
GGTATAG163250.065.617115
CTATACC53250.065.56566
GTATAGC84850.064.566176
GTATACG81350.064.5058066
GTATACC51200.064.227796
TATACGG100550.064.190677
TATAGCG89600.064.148597
GGTATAA164350.064.00725
AGTATAG122850.063.9871865
GGTATAC84850.063.4941865
TTATAGC112150.063.4166346
GCTATAG99650.063.1566355
CGCTATA80400.062.9618644
TATACCC41700.062.919147
CGTATAA139200.062.905225
CTATAGC67600.062.877426
Produced by FastQC (version 0.11.5)