FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_77-BBX31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_77-BBX31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13299186
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG302530.22748008787906265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC84100.066.1395
CGTATAG120900.065.9438865
GTATAGC121100.065.228336
GGTATAG239750.065.150745
GTATAGG253500.064.639126
CGTATAA196300.063.9245385
CTATACC76650.063.6676146
CCTATAC77300.063.6300625
GGTATAA269550.063.5805935
GCTATAG138700.063.4838525
TATAGCG124450.063.472257
AGTATAG179050.063.2448125
TATACGG130100.063.1626857
GTATACG114950.063.148886
GGTATAC117800.062.9871445
CGCTATA109900.062.644644
GGGTATA228900.062.3549424
GCTATAA208750.062.223585
CGGTATA173300.062.214354
TTATAGC163650.062.056546
Produced by FastQC (version 0.11.5)