FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_76-LBD37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_76-LBD37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10538393
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG161260.15302143315399228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC70500.066.1023255
GTATAGG212050.065.683396
GGTATAG196100.065.6199345
CGTATAG99700.065.516525
CTATACC65400.065.479496
GTATAGC101050.065.3336946
CCTATAC65600.064.693195
GCTATAG118550.064.542955
GGTATAA213200.064.393715
GTATACG96900.063.8351866
CGTATAA153550.063.7984125
TATAGCG99850.063.63087
AGTATAG150500.063.3710635
GGTATAC98400.063.146535
CTATAGC77250.062.817356
CGCTATA91150.062.7186974
GCTATAC84150.062.6557245
TTATAGC133450.062.606216
CGGTATA132500.062.5533874
GTATACC59500.062.3878566
Produced by FastQC (version 0.11.5)