FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_75-E2F3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_75-E2F3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11225993
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG261190.2326653864829597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC72300.066.43265
GTATAGC103100.065.8591166
CGTATAG103750.065.648815
GGTATAG206500.065.28915
GTATAGG215750.064.370776
CGTATAA171700.064.178465
CCTATAC67350.064.095365
GGTATAC105000.063.8344655
TATAGCG108150.063.7536777
GCTATAG121250.063.67515
GGTATAA224200.063.4893765
AGTATAG152150.063.2280435
GTATACC62800.063.1687746
TATACGG117350.063.1375927
GTATACG102250.063.0537646
CTATACC67950.062.8601266
CGCTATA97950.062.8218084
CTATAGC81600.062.3766256
GGGTATA201100.062.293294
CGGTATA144800.062.137174
Produced by FastQC (version 0.11.5)