FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_74-BZR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_74-BZR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7471666
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG194020.2596743483983358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC50400.067.124125
CGTATAG68250.066.484445
GGTATAG137100.065.632085
GTATAGC70100.065.528376
GTATAGG149400.065.12286
CCTATAC45750.064.540755
CTATACC43450.064.0923166
GGTATAC71650.063.8666655
GCTATAA119000.063.8256955
CGTATAA115300.063.6285175
GGTATAA151850.063.6112985
TATAGCG73150.063.6092077
AGTATAG101350.063.5841035
GTATACG68000.063.384716
GCTATAG81150.063.330945
CTATACG49600.062.9166876
TATACGG78600.062.8484887
TATACCG50100.062.7077567
CGCTATA62600.062.7048574
GCTATAC57550.062.492875
Produced by FastQC (version 0.11.5)