FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_73-BBX31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_73-BBX31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3927878
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG77430.1971293405752419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG37500.066.3220145
CGTATAC27700.066.171495
GTATAGC35600.065.636576
GGTATAG67400.065.4447255
GTATAGG73600.064.779486
CCTATAC24250.064.767045
GCTATAG43350.063.9887355
CGTATAA60300.063.6948665
GGTATAA74000.063.6257325
GTATACC24250.063.468816
CTATACC24100.063.4284066
GTATACG38350.063.3014346
GGTATAC37850.063.213475
TATACGG42750.063.085887
AGTATAG52800.063.0036965
TTATAGC49200.062.850196
GCTATAA63500.062.5232545
CGGTATA53350.062.3541764
CTATAGC31250.062.3482976
TATACCG27600.062.2280657
Produced by FastQC (version 0.11.5)